5AYF

Crystal structure of SET7/9 in complex with cyproheptadine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-16
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	102.463, 39.366, 67.275
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.616 - 2.005
R-factor	0.1973
Rwork	0.194
R-free	0.25810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1n6a
RMSD bond length	0.008
RMSD bond angle	1.085
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge		0.487
Number of reflections	18892
<I/σ(I)>	17.6	4.2
Completeness [%]	99.9	100
Redundancy	7.2	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	0.1M Tris-HCl pH 8.5, 31% PEG 6000