5AXK

Crystal structure of Thg1 like protein (TLP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-20
Detector	RAYONIX MX225HE
Wavelength(s)	0.978
Spacegroup name	C 2 2 21
Unit cell lengths	98.255, 120.480, 157.392
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.896 - 2.290
R-factor	0.2084
Rwork	0.206
R-free	0.23950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wbz
RMSD bond length	0.009
RMSD bond angle	1.109
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.430
High resolution limit [Å]	2.290	2.290
Rmerge		0.700
Number of reflections	41650
<I/σ(I)>	14.7	2.8
Completeness [%]	98.3	93.8
Redundancy	6.7	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	PEG 3350, tri-potassium citrate