5AWZ
Crystal Structure of the Cell-Free Synthesized Membrane Protein, Acetabularia Rhodopsin I, at 1.57 angstrom
Replaces: 3WT8Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-26 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 75.990, 101.630, 43.760 |
Unit cell angles | 90.00, 119.17, 90.00 |
Refinement procedure
Resolution | 30.540 - 1.570 |
R-factor | 0.1776 |
Rwork | 0.177 |
R-free | 0.19410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c3w |
RMSD bond length | 0.006 |
RMSD bond angle | 1.122 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1690) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.540 | 1.630 |
High resolution limit [Å] | 1.570 | 1.570 |
Number of reflections | 39721 | |
<I/σ(I)> | 9.87 | 1.82 |
Completeness [%] | 98.3 | 83.67 |
Redundancy | 3.8 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 6.5 | 293 | 18% PEG400, 0.1M sodium cacodylate, 0.2M NaCl, 10mM MgCl2, 2.5% MPD |