Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

5AWZ

Crystal Structure of the Cell-Free Synthesized Membrane Protein, Acetabularia Rhodopsin I, at 1.57 angstrom

Replaces:  3WT8
Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSPRING-8 BEAMLINE BL32XU
Synchrotron siteSPring-8
BeamlineBL32XU
Temperature [K]100
Detector technologyCCD
Collection date2010-07-26
DetectorRAYONIX MX225HE
Wavelength(s)1.0
Spacegroup nameC 1 2 1
Unit cell lengths75.990, 101.630, 43.760
Unit cell angles90.00, 119.17, 90.00
Refinement procedure
Resolution30.540 - 1.570
R-factor0.1776
Rwork0.177
R-free0.19410
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1c3w
RMSD bond length0.006
RMSD bond angle1.122
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwareMOLREP
Refinement softwarePHENIX (1.9_1690)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.5401.630
High resolution limit [Å]1.5701.570
Number of reflections39721
<I/σ(I)>9.871.82
Completeness [%]98.383.67
Redundancy3.83.6
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1LIPIDIC CUBIC PHASE6.529318% PEG400, 0.1M sodium cacodylate, 0.2M NaCl, 10mM MgCl2, 2.5% MPD

226707

PDB entries from 2024-10-30

PDB statisticsPDBj update infoContact PDBjnumon