5AVA
Crystal structure of PHA-E lectin in complex with bisected glycan
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2014-02-08 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.210, 122.838, 97.664 |
| Unit cell angles | 90.00, 90.66, 90.00 |
Refinement procedure
| Resolution | 100.000 - 3.000 |
| R-factor | 0.23097 |
| Rwork | 0.229 |
| R-free | 0.27371 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wcs |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.053 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.320 | |
| Number of reflections | 45064 | |
| <I/σ(I)> | 27.1 | 5.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 0.1M sodium acetate (pH 4.5), 25 %(w/v) PEG 3350 |
