5ARL
crystal structure of porcine RNase 4 D80A mutant in complex with dCMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-02 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 30.962, 62.515, 70.216 |
| Unit cell angles | 89.78, 79.21, 78.90 |
Refinement procedure
| Resolution | 68.940 - 2.610 |
| R-factor | 0.25486 |
| Rwork | 0.252 |
| R-free | 0.29958 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5arj |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.514 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.500 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.100 | 0.410 |
| Number of reflections | 14168 | |
| <I/σ(I)> | 27 | 10.7 |
| Completeness [%] | 91.5 | 65.6 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 0.1 M SODIUM CITRATE PH 5.6, 20 % (V/V) 2-PROPANOL, 20 % (W/V) PEG 4000 |
