5ARJ
crystal structure of porcine RNase 4 D80A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-31 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 30.832, 61.821, 68.887 |
| Unit cell angles | 92.04, 100.99, 101.24 |
Refinement procedure
| Resolution | 67.430 - 2.900 |
| R-factor | 0.20998 |
| Rwork | 0.207 |
| R-free | 0.28154 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5AR6. |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.279 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.390 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.140 | 0.200 |
| Number of reflections | 7962 | |
| <I/σ(I)> | 8 | 2 |
| Completeness [%] | 73.5 | 75.5 |
| Redundancy | 1.6 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M SODIUM CACODYLATE PH 6.5, 1.0 M SODIUM CITRATE TRIBASIC DIHYDRATE |
