5AP4
Naturally Occurring Mutations in the MPS1 Gene Predispose Cells to Kinase Inhibitor Drug Resistance.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-05-23 |
Detector | DECTRIS PILATUS 2M |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 70.420, 112.080, 113.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.860 - 2.850 |
R-factor | 0.1978 |
Rwork | 0.196 |
R-free | 0.22630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4c4j |
RMSD bond length | 0.010 |
RMSD bond angle | 1.190 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.860 | 3.000 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.090 | 0.850 |
Number of reflections | 10484 | |
<I/σ(I)> | 10.8 | 1.5 |
Completeness [%] | 97.1 | 99 |
Redundancy | 3.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 38% (V/V) PEG300, PH 7.5 |