5AMH
Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with Thalidomide, trigonal crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-06-24 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 51.720, 51.720, 84.710 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.770 - 1.200 |
R-factor | 0.12716 |
Rwork | 0.126 |
R-free | 0.15737 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4v2y |
RMSD bond length | 0.023 |
RMSD bond angle | 2.398 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.800 | 1.270 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.060 | 0.740 |
Number of reflections | 41611 | |
<I/σ(I)> | 11.85 | 1.78 |
Completeness [%] | 99.7 | 99.3 |
Redundancy | 3.91 | 3.69 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 100 MM SODIUM ACETATE PH 4.6, 2.2 M CALCIUM CHLORIDE |