5AJJ
Crystal structure of variola virus virulence factor F1L in complex with human Bid BH3 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-17 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 52.094, 112.318, 117.178 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.828 - 1.750 |
| R-factor | 0.1689 |
| Rwork | 0.167 |
| R-free | 0.20830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d2m |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.150 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: DEV_1760)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.060 | 0.460 |
| Number of reflections | 17569 | |
| <I/σ(I)> | 39 | 3.6 |
| Completeness [%] | 97.1 | 80.4 |
| Redundancy | 13.3 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 1.8 M SODIUM ACETATE AND 0.1 M HEPES PH 6.5 |
