5AJD
Not1 C-terminal domain in complex with Not4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-19 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 173.658, 173.658, 262.612 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 75.655 - 3.620 |
| R-factor | 0.2688 |
| Rwork | 0.266 |
| R-free | 0.31870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4by6 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.605 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.660 | 3.750 |
| High resolution limit [Å] | 3.620 | 3.620 |
| Rmerge | 0.110 | 1.500 |
| Number of reflections | 52676 | |
| <I/σ(I)> | 11.26 | 1.37 |
| Completeness [%] | 99.8 | 98.6 |
| Redundancy | 6.7 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 10% (W/V) PEG 4000, 0.02 M OF 1, 6-HEXANEDIOL, 0.02 M OF 1-BUTANOL, 0.02 M OF 1, 2-PROPANEDIOL (RACEMIC), 0.02 M OF 2-PROPANOL, 0T.02 M OF 1,4- BUTANEDIOL, 0.02 M OF 1,3-PROPANEDIOL, 0.1 M MOPS/HEPES-NA 7.5 AND 20% GLYCEROL |
