5AHM
IMP-bound form of the DeltaCBS mutant of IMPDH from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-12-16 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | I 4 |
Unit cell lengths | 103.600, 103.600, 158.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.300 - 1.740 |
R-factor | 0.1622 |
Rwork | 0.161 |
R-free | 0.18870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4dqw |
RMSD bond length | 0.009 |
RMSD bond angle | 1.109 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.340 | 1.810 |
High resolution limit [Å] | 1.750 | 1.740 |
Rmerge | 0.040 | 0.440 |
Number of reflections | 152733 | |
<I/σ(I)> | 13.1 | 2.9 |
Completeness [%] | 99.5 | 98.9 |
Redundancy | 3.5 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | HEPES 0.1 M PH 7.5, NH4SO4 1.26 M |