5AHK
Crystal structure of acetohydroxy acid synthase Pf5 from Pseudomonas protegens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-26 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.220, 139.870, 65.340 |
| Unit cell angles | 90.00, 97.87, 90.00 |
Refinement procedure
| Resolution | 49.220 - 1.550 |
| R-factor | 0.15227 |
| Rwork | 0.151 |
| R-free | 0.17390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ybh |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.422 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.200 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.070 | 0.580 |
| Number of reflections | 151815 | |
| <I/σ(I)> | 10 | 2.3 |
| Completeness [%] | 90.9 | 81.6 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 10% (W/V) PEG 4000, 0.1 M MES/IMIDAZOLE PH 6.5, 0.3 M MGCL2, 0.3 M CACL2, 17% (V/V) GLYCEROL |
