5AH5
Crystal structure of the ternary complex of Agrobacterium radiobacter K84 agnB2 LeuRS-tRNA-LeuAMS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-18 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 169.910, 50.320, 170.520 |
Unit cell angles | 90.00, 93.48, 90.00 |
Refinement procedure
Resolution | 48.580 - 2.100 |
R-factor | 0.19634 |
Rwork | 0.194 |
R-free | 0.23732 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 2BTE 4as1 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.736 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.600 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.080 | 0.760 |
Number of reflections | 160330 | |
<I/σ(I)> | 10.6 | 2.1 |
Completeness [%] | 99.4 | 99.6 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 0.1 M MES PH 6.5, 0.2 M AMMONIUM SULFATE, 27 % PEG 5000 MME |