5AH4
The sliding clamp of Mycobacterium smegmatis in complex with a natural product.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-08-22 |
Detector | DECTRIS PILATUS 2M-F |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 151.888, 96.124, 92.272 |
Unit cell angles | 90.00, 124.41, 90.00 |
Refinement procedure
Resolution | 76.271 - 2.313 |
R-factor | 0.1755 |
Rwork | 0.173 |
R-free | 0.22330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3p16 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.173 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 76.270 | 2.320 |
High resolution limit [Å] | 2.310 | 2.310 |
Rmerge | 0.070 | 0.860 |
Number of reflections | 47835 | |
<I/σ(I)> | 19.5 | 2.2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 200 MM MGCL2 16.3 % (V/V) GLYCEROL 12.2 % (W/V) PEG 3350 100 MM TRIS-HCL PH 7.44 |