5AH2
The sliding clamp of Mycobacterium smegmatis in complex with a natural product.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-25 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.241, 125.868, 94.899 |
| Unit cell angles | 90.00, 104.42, 90.00 |
Refinement procedure
| Resolution | 77.712 - 2.129 |
| R-factor | 0.1845 |
| Rwork | 0.182 |
| R-free | 0.23140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3p16 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.212 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER (via) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 125.870 | 2.140 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.110 | 0.930 |
| Number of reflections | 102042 | |
| <I/σ(I)> | 13.6 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.9 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 370 MM MGCL2, 7.33 % (V/V) GLYCEROL, 25.6 % (W/V) JEFFAMINE M-2070, 100 MM TRIS-HCL PH 8.56 |
