5AGY
CRYSTAL STRUCTURE OF A TAU CLASS GST MUTANT FROM GLYCINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-17 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.241, 77.602, 99.078 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.794 - 1.750 |
| R-factor | 0.1675 |
| Rwork | 0.167 |
| R-free | 0.18740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oyj |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.002 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.790 | 1.850 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.050 | 0.450 |
| Number of reflections | 48837 | |
| <I/σ(I)> | 23 | |
| Completeness [%] | 99.3 | 97.7 |
| Redundancy | 6.95 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | PEG 4K 27.5%, NH4OAC 0.2 M, NAOAC 0.1 M, PH 5.0, 10 MM GTB |
