5AGY
CRYSTAL STRUCTURE OF A TAU CLASS GST MUTANT FROM GLYCINE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-17 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.241, 77.602, 99.078 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.794 - 1.750 |
R-factor | 0.1675 |
Rwork | 0.167 |
R-free | 0.18740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1oyj |
RMSD bond length | 0.014 |
RMSD bond angle | 1.002 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.790 | 1.850 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.050 | 0.450 |
Number of reflections | 48837 | |
<I/σ(I)> | 23 | |
Completeness [%] | 99.3 | 97.7 |
Redundancy | 6.95 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5 | PEG 4K 27.5%, NH4OAC 0.2 M, NAOAC 0.1 M, PH 5.0, 10 MM GTB |