5AGW

Bcl-2 alpha beta-1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-12
Detector	ADSC CCD
Spacegroup name	P 43 21 2
Unit cell lengths	87.673, 87.673, 107.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.969 - 2.695
R-factor	0.2051
Rwork	0.202
R-free	0.27190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	UNPUBLISHED BCL-2 BECLIN BH3 COMPLEX
RMSD bond length	0.009
RMSD bond angle	1.274
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.970	2.790
High resolution limit [Å]	2.700	2.700
Rmerge	0.100
Number of reflections	12081
<I/σ(I)>	18.29	1.08
Completeness [%]	99.8	98.3
Redundancy	13.4	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		0.02M CACL2, 0.1M MOPS PH7.0, 10% 2-PROPANOL