5AGJ
Crystal structure of the LeuRS editing domain of Candida albicans in complex with the adduct AN2690-AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-17 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.950, 59.260, 97.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.570 - 2.000 |
| R-factor | 0.18141 |
| Rwork | 0.179 |
| R-free | 0.23541 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wfg |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.496 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.570 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.130 | 0.550 |
| Number of reflections | 16451 | |
| <I/σ(I)> | 9.73 | 3 |
| Completeness [%] | 99.7 | 97 |
| Redundancy | 4 | 3.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 0.2 M SODIUM ACETATE, 0.1 M SODIUM CACODYLATE PH 6.5, 30 % PEG 8000 |
