5AF2
Crystal structure of the C-terminal 2',5'-phosphodiesterase domain of group A rotavirus protein VP3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-04 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.290, 74.960, 63.220 |
| Unit cell angles | 90.00, 112.80, 90.00 |
Refinement procedure
| Resolution | 45.119 - 1.390 |
| R-factor | 0.1717 |
| Rwork | 0.171 |
| R-free | 0.19280 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.122 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.120 | 1.430 |
| High resolution limit [Å] | 1.390 | 1.390 |
| Rmerge | 0.070 | 0.620 |
| Number of reflections | 105693 | |
| <I/σ(I)> | 20.1 | 3.7 |
| Completeness [%] | 99.7 | 98.4 |
| Redundancy | 13.3 | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1 M TRIS-ACETATE PH 7.0, 0.2 M KSCN AND 22 % PEG MONOMETHYL ETHER 2000 |
