5AF2
Crystal structure of the C-terminal 2',5'-phosphodiesterase domain of group A rotavirus protein VP3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-10-04 |
Detector | DECTRIS PILATUS |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.290, 74.960, 63.220 |
Unit cell angles | 90.00, 112.80, 90.00 |
Refinement procedure
Resolution | 45.119 - 1.390 |
R-factor | 0.1717 |
Rwork | 0.171 |
R-free | 0.19280 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.006 |
RMSD bond angle | 1.122 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.120 | 1.430 |
High resolution limit [Å] | 1.390 | 1.390 |
Rmerge | 0.070 | 0.620 |
Number of reflections | 105693 | |
<I/σ(I)> | 20.1 | 3.7 |
Completeness [%] | 99.7 | 98.4 |
Redundancy | 13.3 | 11.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 0.1 M TRIS-ACETATE PH 7.0, 0.2 M KSCN AND 22 % PEG MONOMETHYL ETHER 2000 |