5AE8
Crystal structure of mouse PI3 kinase delta in complex with GSK2269557
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-10 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 142.958, 64.398, 116.795 |
| Unit cell angles | 90.00, 103.17, 90.00 |
Refinement procedure
| Resolution | 47.170 - 2.420 |
| R-factor | 0.1926 |
| Rwork | 0.190 |
| R-free | 0.25030 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 114.000 | 2.560 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.060 | 0.500 |
| Number of reflections | 39079 | |
| <I/σ(I)> | 13.8 | 2 |
| Completeness [%] | 98.8 | 97.2 |
| Redundancy | 3.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
