5ADP
Crystal structure of the A.17 antibody FAB fragment - Light chain S35R mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 10 |
| Detector technology | CCD |
| Collection date | 2011-12-08 |
| Detector | MARRESEARCH MAR 165 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.547, 67.040, 66.950 |
| Unit cell angles | 90.00, 107.63, 90.00 |
Refinement procedure
| Resolution | 19.360 - 2.130 |
| R-factor | 0.181 |
| Rwork | 0.179 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xza |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.800 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.420 | 2.200 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.040 | 0.270 |
| Number of reflections | 24223 | |
| <I/σ(I)> | 19.1 | 4.5 |
| Completeness [%] | 97.7 | 92.2 |
| Redundancy | 3.2 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.15 M MGCL2 18% (W/V) POLYETHYLENE GLYCOL 6000 0.1 M N-(2-ACETAMIDO) IMINODIACETIC ACID (ADA) PH 7.5 |
