5AAW
Structure of a redesigned cross-reactive antibody to dengue virus with increased in vivo potency
Replaces: 4UDZExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-20 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 87.110, 131.670, 176.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.750 - 3.270 |
| R-factor | 0.22847 |
| Rwork | 0.227 |
| R-free | 0.26258 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4UD3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.494 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 105.530 | 3.450 |
| High resolution limit [Å] | 3.270 | 3.270 |
| Rmerge | 0.150 | 0.490 |
| Number of reflections | 31554 | |
| <I/σ(I)> | 4.9 | 1.5 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 5.4 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
