5AAM
Structure of a redesigned cross-reactive antibody to dengue virus with increased in vivo potency
Replaces: 4UD3Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-29 |
Detector | DECTRIS PILATUS |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 71.638, 55.891, 87.047 |
Unit cell angles | 90.00, 104.58, 90.00 |
Refinement procedure
Resolution | 29.550 - 2.490 |
R-factor | 0.2222 |
Rwork | 0.220 |
R-free | 0.26980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3uyp |
RMSD bond length | 0.010 |
RMSD bond angle | 1.230 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | BUSTER-TNT (BUSTER 2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.900 | 2.630 |
High resolution limit [Å] | 2.490 | 2.490 |
Rmerge | 0.080 | 1.150 |
Number of reflections | 23183 | |
<I/σ(I)> | 14.8 | 1.4 |
Completeness [%] | 98.3 | 92.3 |
Redundancy | 6.8 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |