5A9K
Structural basis for DNA strand separation by a hexameric replicative helicase
EM specimen
Staining | Yes |
Staining material | uranyl acetate |
EM Data Collection
Microscope | FEI TECNAI F20 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Cs (mm) | 2.1 |
Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
Number of Images | 30 |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 19 |
Software | CTFFIND,ctfit,UCSF Chimera,IMAGIC |
Symmetry | C6 |