5A9J
Crystal structure of the Helicase domain of human DNA polymerase theta, apo-form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-18 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 128.017, 130.531, 160.910 |
| Unit cell angles | 90.00, 100.77, 90.00 |
Refinement procedure
| Resolution | 48.861 - 3.550 |
| R-factor | 0.2269 |
| Rwork | 0.225 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5aga |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.981 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.800 | 3.640 |
| High resolution limit [Å] | 3.550 | 3.550 |
| Rmerge | 0.050 | 0.740 |
| Number of reflections | 62574 | |
| <I/σ(I)> | 12.8 | 1.8 |
| Completeness [%] | 99.3 | 99.4 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M NACL, 20% PEG 6K, 10% ETHYLENE GLYCOL, 0.1 M HEPES PH 7.0 |
