5A9J
Crystal structure of the Helicase domain of human DNA polymerase theta, apo-form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-01-18 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 128.017, 130.531, 160.910 |
Unit cell angles | 90.00, 100.77, 90.00 |
Refinement procedure
Resolution | 48.861 - 3.550 |
R-factor | 0.2269 |
Rwork | 0.225 |
R-free | 0.26500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5aga |
RMSD bond length | 0.004 |
RMSD bond angle | 0.981 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.800 | 3.640 |
High resolution limit [Å] | 3.550 | 3.550 |
Rmerge | 0.050 | 0.740 |
Number of reflections | 62574 | |
<I/σ(I)> | 12.8 | 1.8 |
Completeness [%] | 99.3 | 99.4 |
Redundancy | 3.4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.2M NACL, 20% PEG 6K, 10% ETHYLENE GLYCOL, 0.1 M HEPES PH 7.0 |