5A97
Hazara virus nucleocapsid protain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2013-06-23 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.989, 76.102, 449.282 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 224.640 - 2.700 |
| R-factor | 0.24384 |
| Rwork | 0.242 |
| R-free | 0.28217 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4akl |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.098 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.250 | 2.770 |
| High resolution limit [Å] | 2.600 | 2.700 |
| Rmerge | 0.110 | 0.630 |
| Number of reflections | 74079 | |
| <I/σ(I)> | 15.1 | 1.7 |
| Completeness [%] | 100.0 | 99 |
| Redundancy | 10.5 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 15% PEG 10,000, 0.1 M SODIUM CITRATE PH 5, 0.1 M NACL, 2 % DIOXANE |
