5A7J
Crystal structure of INPP5B in complex with benzene 1,2,4,5- tetrakisphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.261, 94.442, 78.313 |
| Unit cell angles | 90.00, 106.32, 90.00 |
Refinement procedure
| Resolution | 48.480 - 2.900 |
| R-factor | 0.2022 |
| Rwork | 0.200 |
| R-free | 0.25030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3n9v |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (BUSTER 2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.480 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.160 | 0.580 |
| Number of reflections | 16223 | |
| <I/σ(I)> | 6.9 | 2.3 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 0.2 M LI2SO4, 0.1 M BIS-TRIS PH 5.5 AND 25% PEG3350 |
