5A5T
Structure of mammalian eIF3 in the context of the 43S preinitiation complex
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI/PHILIPS CM300FEG/HE |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2 |
| Detector | GATAN K2 (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 6 |
| Software | RELION |
| Symmetry | C1 |
