5A4P
Structure of UBE2Z provides functional insight into specificity in the FAT10 conjugation machinery
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-11 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 45.309, 57.800, 105.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.880 - 2.100 |
| R-factor | 0.2372 |
| Rwork | 0.235 |
| R-free | 0.27820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x23 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.890 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.100 | 0.590 |
| Number of reflections | 16612 | |
| <I/σ(I)> | 8 | 1.6 |
| Completeness [%] | 94.3 | 95.2 |
| Redundancy | 2.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 17% (W/V) PEG1500, 0.1M MMT PH 6.5 |
