5A43
Crystal structure of a dual topology fluoride ion channel.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-20 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 41 |
| Unit cell lengths | 87.420, 87.420, 146.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.246 - 2.580 |
| R-factor | 0.2258 |
| Rwork | 0.224 |
| R-free | 0.26420 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.446 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX (SHARP) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.170 | 0.990 |
| Number of reflections | 34593 | |
| <I/σ(I)> | 9 | 1.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 14.3 | 14.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 29% PEG550 MME, 50 MM LINO3, 100 MM ADA PH 6.0 |
