5A43
Crystal structure of a dual topology fluoride ion channel.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-20 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 41 |
Unit cell lengths | 87.420, 87.420, 146.810 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.246 - 2.580 |
R-factor | 0.2258 |
Rwork | 0.224 |
R-free | 0.26420 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.010 |
RMSD bond angle | 1.446 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELX (SHARP) |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.170 | 0.990 |
Number of reflections | 34593 | |
<I/σ(I)> | 9 | 1.9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 14.3 | 14.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 29% PEG550 MME, 50 MM LINO3, 100 MM ADA PH 6.0 |