5A3N
Crystal structure of human PLU-1 (JARID1B) in complex with KDOAM25a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-06 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 141.874, 141.874, 151.865 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 122.870 - 2.000 |
| R-factor | 0.18382 |
| Rwork | 0.183 |
| R-free | 0.20335 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 51AF |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.358 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | CCP4-AUTOMR |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.810 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.640 |
| Number of reflections | 60715 | |
| <I/σ(I)> | 21.7 | 3.5 |
| Completeness [%] | 99.3 | 95.8 |
| Redundancy | 12.6 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1M HEPES PH 8.0, 0.8M POTASSIUM PHOSPHATE-DIBASIC, 0.8M SODIUM PHOSPHATE MONOBASIC |
