5A1T
Trichomonas vaginalis lactate dehydrogenase in complex with NADH and oxamate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OXFORD ENHANCE ULTRA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-07 |
| Detector | OXFORD |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 80.120, 82.680, 114.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.998 - 1.970 |
| R-factor | 0.2037 |
| Rwork | 0.201 |
| R-free | 0.25030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uul |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.812 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 2.020 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.020 | 1.500 |
| Number of reflections | 39768 | |
| <I/σ(I)> | 14.7 | 1.3 |
| Completeness [%] | 73.1 | 54.4 |
| Redundancy | 13 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M POTASSIUM SODIUM TARTRATE TETRAHYDRATE, 0.1 M SODIUM CITRATE TRIBASIC DIHYDRATE PH 5.6, 2.0 M AMMONIUM SULFATE |
