5A1G
The structure of Human MAT2A in complex with S-adenosylethionine and PPNP.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-11 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 68.390, 94.390, 117.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.690 - 1.830 |
| R-factor | 0.12248 |
| Rwork | 0.120 |
| R-free | 0.17490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p02 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.376 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.340 | 1.900 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.100 | 0.810 |
| Number of reflections | 32401 | |
| <I/σ(I)> | 21.5 | 3.4 |
| Completeness [%] | 99.3 | 97.5 |
| Redundancy | 13.3 | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1 M IMIDAZOLE PH 7.0, 50 % MPD |
