5A1F
Crystal structure of the catalytic domain of PLU1 in complex with N-oxalylglycine.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2015-03-06 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 141.855, 141.855, 152.129 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.010 - 2.100 |
| R-factor | 0.2006 |
| Rwork | 0.199 |
| R-free | 0.22501 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gio |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.503 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | CCP4I |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.770 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.090 | 0.090 |
| Number of reflections | 52970 | |
| <I/σ(I)> | 19.14 | 2.42 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.5 | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1M HEPES PH 8.0, 0.8M POTASSIUM PHOSPHATE-DIBASIC, 0.8M SODIUM PHOSPHATE MONOBASIC |
