5A19
The structure of MAT2A in complex with PPNP.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-08-11 |
Detector | DECTRIS PILATUS 2M |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 66.220, 95.490, 117.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.710 - 2.340 |
R-factor | 0.16549 |
Rwork | 0.163 |
R-free | 0.20446 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2p02 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.276 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.040 | 2.400 |
High resolution limit [Å] | 2.330 | 2.340 |
Rmerge | 0.130 | 0.600 |
Number of reflections | 16225 | |
<I/σ(I)> | 20.7 | 1.7 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 13 | 13.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.1 M HEPES PH 7.5, 30 % PEG 600 |