5A0T
Catalysis and 5' end sensing by ribonuclease RNase J of the metallo- beta-lactamase family
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-26 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 186.187, 186.187, 113.382 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.340 - 2.283 |
| R-factor | 0.1463 |
| Rwork | 0.145 |
| R-free | 0.17690 |
| Structure solution method | SAD |
| Starting model (for MR) | 3bk1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.206 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | SHELX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.300 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.050 | 0.490 |
| Number of reflections | 88741 | |
| <I/σ(I)> | 10.6 | 2.5 |
| Completeness [%] | 99.3 | 97.9 |
| Redundancy | 8.2 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | THE BEST CRYSTALS OF S. COELICOLOR RNASE J WERE OBTAINED IN THE 27.5% W/V PEG 400, 0.1 M TRIS-HCL, PH 8.5 BY MIXING A 1:2 VOLUME RATIO OF CRYSTALLIZATION RESERVOIR TO PROTEIN SOLUTION. |
