5ZXM

Crystal Structure of GyraseB N-terminal at 1.93A Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	273
Detector technology	PIXEL
Collection date	2018-02-09
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.96
Spacegroup name	P 2 21 21
Unit cell lengths	79.420, 86.530, 135.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.004 - 1.938
R-factor	0.1824
Rwork	0.181
R-free	0.22550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ei1
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	REFMAC (5.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.004	1.970
High resolution limit [Å]	1.938	1.940
Rmerge	0.101	0.800
Number of reflections	69892	3436
<I/σ(I)>	10.3
Completeness [%]	99.3
Redundancy	4.5
CC(1/2)	0.990	0.670

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	PEG 3350, lithium sulfate, ATP, Tris, pH 8.0