5ZWJ

Crystal structure of EGFR 675-1022 T790M/C797S/V948R in complex with EAI045

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-20
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.91905
Spacegroup name	P 61 2 2
Unit cell lengths	79.586, 79.586, 215.075
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.686 - 2.900
R-factor	0.2559
Rwork	0.255
R-free	0.27180
RMSD bond length	0.016
RMSD bond angle	1.392
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.000
High resolution limit [Å]	2.900	2.900
Number of reflections	10084
<I/σ(I)>	20.1
Completeness [%]	99.9
Redundancy	17.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	26% PEG 4000, 0.1 M KCl, 0.05 M Tris pH 7.5-8.0