5ZVB

APOBEC3F Chimeric Catalytic Domain in Complex with DNA(dT9)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-06
Detector	MAR CCD 130 mm
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	60.990, 92.702, 93.674
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 2.000
R-factor	0.1742
Rwork	0.172
R-free	0.22030
Structure solution method	SAD
Data reduction software	ADDREF
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.010	2.020
High resolution limit [Å]	1.980	1.980
Rmerge	0.146	0.662
Number of reflections	37457
<I/σ(I)>	5.3
Completeness [%]	99.4
Redundancy	14.3
CC(1/2)	0.999	0.936

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.05M sodium cacodylate, pH 6.5, and 30%(v/v) 2-methyl-2,4-pentanediol (MPD)