5ZQK

Dengue Virus Non Structural Protein 5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980105
Spacegroup name	P 1 21 1
Unit cell lengths	86.680, 146.100, 97.240
Unit cell angles	90.00, 105.45, 90.00

Refinement procedure

Resolution	46.000 - 2.300
R-factor	0.198
Rwork	0.196
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3evg 4k6m
RMSD bond length	0.010
RMSD bond angle	1.140
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.000	2.440
High resolution limit [Å]	2.290	2.290
Number of reflections	103117	16247
<I/σ(I)>	8.62
Completeness [%]	99.7
Redundancy	3.89
CC(1/2)	0.996	0.631

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	PEG 4000, Glycerol, Tris HCl, Bicine