5ZQ8
Crystal structure of spRlmCD with U747 stemloop RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9774 |
| Spacegroup name | P 1 |
| Unit cell lengths | 62.230, 62.259, 79.818 |
| Unit cell angles | 74.58, 85.47, 65.03 |
Refinement procedure
| Resolution | 56.370 - 2.180 |
| R-factor | 0.248 |
| Rwork | 0.245 |
| R-free | 0.30330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xj1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.318 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 56.370 | 56.370 | 2.290 |
| High resolution limit [Å] | 2.180 | 6.880 | 2.180 |
| Rmerge | 0.130 | 0.081 | 0.551 |
| Rmeas | 0.152 | 0.095 | 0.647 |
| Rpim | 0.078 | 0.049 | 0.338 |
| Total number of observations | 176570 | 6122 | 19299 |
| Number of reflections | 48209 | 1721 | 5469 |
| <I/σ(I)> | 6.2 | 11.6 | 2.2 |
| Completeness [%] | 87.8 | 98.6 | 67.9 |
| Redundancy | 3.7 | 3.6 | 3.5 |
| CC(1/2) | 0.991 | 0.990 | 0.891 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M Ammonium acetate, 0.1M sodium acetate, pH5.2, 20%(w/v) PEG3350 |
