5ZOL
Crystal structure of a three sites mutantion of FSAA complexed with HA and product
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-05 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 103.085, 103.085, 492.895 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.700 - 2.172 |
| R-factor | 0.1929 |
| Rwork | 0.191 |
| R-free | 0.23200 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.904 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.210 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Number of reflections | 161347 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 16.3 | 15.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Hepes pH7.2, 18% PEG 4000, 10% isopropyl alcohol, 0.2M ammonium sulfate, 0.01M disodium malonate |
