5ZOG

Crystal Structure of R192F hFen1 in complex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	95
Detector technology	CCD
Collection date	2016-12-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	C 2 2 21
Unit cell lengths	88.330, 244.800, 70.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.559 - 2.299
R-factor	0.2098
Rwork	0.209
R-free	0.23130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3q8k
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.559	29.559	2.360
High resolution limit [Å]	2.299	10.280	2.300
Rmerge	0.071	0.020	0.713
Rmeas	0.079	0.023	0.798
Number of reflections	34330	384	2316
<I/σ(I)>	16.12	44.24	2.53
Completeness [%]	98.8	84.6	90.7
Redundancy	5.143	4.341	5.094
CC(1/2)	0.998	0.999	0.776

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.8	298	PEG 3350, MgCl2, Tris, KCl