5ZOG

Crystal Structure of R192F hFen1 in complex with DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Flap endonuclease 1	polymer	344	38907.7	1	UniProt (P39748) Pfam (PF00752) Pfam (PF00867) In PDB	Homo sapiens (Human)	FEN-1,DNase IV,Flap structure-specific endonuclease 1,Maturation factor 1,hFEN-1
2	B	DNA (5'-D(*CP*CP*CP*GP*TP*CP*C)-3')	polymer	7	2034.3	1		synthetic construct
3	C	DNA (5'-D(P*TP*CP*CP*TP*CP*TP*GP*CP*CP*TP*CP*AP*AP*GP*AP*CP*GP*GP*G)-3')	polymer	19	5781.7	1		synthetic construct
4	D	DNA (5'-D(*TP*GP*AP*GP*GP*CP*AP*GP*AP*GP*GP*AP*T)-3')	polymer	13	4080.7	1		synthetic construct
5		water	water		18.0	85	Chemie (HOH)

Sequence modifications

A: 1 - 333 (UniProt: P39748)

PDB	External Database	Details
Phe 192	Arg 192	engineered mutation
Lys 334	-	expression tag
Leu 335	-	expression tag
Ala 336	-	expression tag
Ala 337	-	expression tag
Ala 338	-	expression tag
Leu 339	-	expression tag
Glu 340	-	expression tag
His 341	-	expression tag
His 342	-	expression tag
His 343	-	expression tag
His 344	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	50804.5
All*	Total formula weight	50804.5

*Water molecules are not included.