5ZOG
Crystal Structure of R192F hFen1 in complex with DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Flap endonuclease 1 | polymer | 344 | 38907.7 | 1 | UniProt (P39748) Pfam (PF00752) Pfam (PF00867) | Homo sapiens (Human) | FEN-1,DNase IV,Flap structure-specific endonuclease 1,Maturation factor 1,hFEN-1 |
| 2 | B (B) | DNA (5'-D(*CP*CP*CP*GP*TP*CP*C)-3') | polymer | 7 | 2034.3 | 1 | synthetic construct | ||
| 3 | C (C) | DNA (5'-D(P*TP*CP*CP*TP*CP*TP*GP*CP*CP*TP*CP*AP*AP*GP*AP*CP*GP*GP*G)-3') | polymer | 19 | 5781.7 | 1 | synthetic construct | ||
| 4 | D (D) | DNA (5'-D(*TP*GP*AP*GP*GP*CP*AP*GP*AP*GP*GP*AP*T)-3') | polymer | 13 | 4080.7 | 1 | synthetic construct | ||
| 5 | E, F, G (A, B, C) | water | water | 18.0 | 85 | Chemie (HOH) |
Sequence modifications
A: 1 - 333 (UniProt: P39748)
| PDB | External Database | Details |
|---|---|---|
| Phe 192 | Arg 192 | engineered mutation |
| Lys 334 | - | expression tag |
| Leu 335 | - | expression tag |
| Ala 336 | - | expression tag |
| Ala 337 | - | expression tag |
| Ala 338 | - | expression tag |
| Leu 339 | - | expression tag |
| Glu 340 | - | expression tag |
| His 341 | - | expression tag |
| His 342 | - | expression tag |
| His 343 | - | expression tag |
| His 344 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 50804.5 | |
| All* | Total formula weight | 50804.5 |
