5ZOG
Crystal Structure of R192F hFen1 in complex with DNA
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Flap endonuclease 1 | polymer | 344 | 38907.7 | 1 | UniProt (P39748) Pfam (PF00752) Pfam (PF00867) In PDB | Homo sapiens (Human) | FEN-1,DNase IV,Flap structure-specific endonuclease 1,Maturation factor 1,hFEN-1 |
2 | B | DNA (5'-D(*CP*CP*CP*GP*TP*CP*C)-3') | polymer | 7 | 2034.3 | 1 | synthetic construct | ||
3 | C | DNA (5'-D(P*TP*CP*CP*TP*CP*TP*GP*CP*CP*TP*CP*AP*AP*GP*AP*CP*GP*GP*G)-3') | polymer | 19 | 5781.7 | 1 | synthetic construct | ||
4 | D | DNA (5'-D(*TP*GP*AP*GP*GP*CP*AP*GP*AP*GP*GP*AP*T)-3') | polymer | 13 | 4080.7 | 1 | synthetic construct | ||
5 | water | water | 18.0 | 85 | Chemie (HOH) |
Sequence modifications
A: 1 - 333 (UniProt: P39748)
PDB | External Database | Details |
---|---|---|
Phe 192 | Arg 192 | engineered mutation |
Lys 334 | - | expression tag |
Leu 335 | - | expression tag |
Ala 336 | - | expression tag |
Ala 337 | - | expression tag |
Ala 338 | - | expression tag |
Leu 339 | - | expression tag |
Glu 340 | - | expression tag |
His 341 | - | expression tag |
His 342 | - | expression tag |
His 343 | - | expression tag |
His 344 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 50804.5 | |
All* | Total formula weight | 50804.5 |