5ZNP

Crystal structure of PtSHL in complex with an H3K4me3 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	44.340, 91.909, 114.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.955 - 2.800
R-factor	0.2515
Rwork	0.249
R-free	0.29320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2f6n 1w4s
RMSD bond length	0.005
RMSD bond angle	1.066
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.136	0.770
Number of reflections	12194	1151
<I/σ(I)>	11.2	1.6
Completeness [%]	99.4	99.6
Redundancy	4.3	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2 M potassium acetate, 20% PEG 3350