5ZLP
Crystal structure of glutamine synthetase from helicobacter pylori
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-05-31 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.95950 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 234.409, 135.167, 203.080 |
Unit cell angles | 90.00, 91.61, 90.00 |
Refinement procedure
Resolution | 47.264 - 2.930 |
R-factor | 0.1716 |
Rwork | 0.168 |
R-free | 0.24610 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.172 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.950 |
High resolution limit [Å] | 2.900 | 7.860 | 2.900 |
Rmerge | 0.148 | 0.086 | 0.367 |
Rmeas | 0.177 | 0.105 | 0.434 |
Rpim | 0.096 | 0.059 | 0.231 |
Total number of observations | 454812 | ||
Number of reflections | 135884 | 6709 | 6838 |
<I/σ(I)> | 8.8 | ||
Completeness [%] | 98.8 | 95.1 | 99.7 |
Redundancy | 3.3 | 3 | 3.4 |
CC(1/2) | 0.982 | 0.886 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 296 | sodium citrate, cosium formate |