5ZL6
Histidine Racemase from Leuconostoc mesenteroides subsp. sake NBRC 102480
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.048, 142.468, 78.701 |
| Unit cell angles | 90.00, 99.30, 90.00 |
Refinement procedure
| Resolution | 33.600 - 2.100 |
| R-factor | 0.177 |
| Rwork | 0.174 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3co8 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.033 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | BALBES |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.600 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.129 | 0.353 |
| Number of reflections | 84173 | 8174 |
| <I/σ(I)> | 8.7 | 2.9 |
| Completeness [%] | 98.2 | 95.8 |
| Redundancy | 3.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10% (w/v) polyethylene glycol 4000, 5% (w/v) glycerol, 200 mM magnesium chloride and a 100 mM Tris-HCl buffer (pH 7.0) |
