5ZJD

Lactate dehydrogenase with NADH and MLA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-11
Detector	ADSC QUANTUM 210
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	65.007, 265.691, 88.006
Unit cell angles	90.00, 103.17, 90.00

Refinement procedure

Resolution	29.803 - 2.394
R-factor	0.1904
Rwork	0.187
R-free	0.25810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4okn
RMSD bond length	0.008
RMSD bond angle	1.050
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.803	2.421
High resolution limit [Å]	2.394	2.394
Rmerge	0.094	0.309
Number of reflections	103853	1212123
<I/σ(I)>	18.3
Completeness [%]	99.9
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	8% Tacsimate (pH 7.0), 20% polyethylene glycol (PEG) 3350