5ZD4

Crystal structure of MBP-fused BIL1/BZR1 in complex with double-stranded DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2017-04-19
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	102.701, 92.603, 111.967
Unit cell angles	90.00, 100.42, 90.00

Refinement procedure

Resolution	43.598 - 2.170
R-factor	0.218
Rwork	0.217
R-free	0.24440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5iqz
RMSD bond length	0.006
RMSD bond angle	0.786
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.330	2.210
High resolution limit [Å]	2.170	2.170
Rmeas	0.061	0.578
Rpim	0.033	0.320
Number of reflections	109100	5420
<I/σ(I)>	21.2	3.5
Completeness [%]	99.9	100
Redundancy	6.8
CC(1/2)	0.999	0.897

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	50mM sodium cacodylate (pH 6.5), 200mM potassium chloride, 10% (w/v) polyethylene glycol (PEG) 4000