5ZBU

Crystal Structure of PA-TM-RING E3 ligase RNF13 RING domain in complex with E2~Ub

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-18
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97947
Spacegroup name	P 1 21 1
Unit cell lengths	56.559, 105.740, 60.790
Unit cell angles	90.00, 115.49, 90.00

Refinement procedure

Resolution	28.258 - 3.200
R-factor	0.2166
Rwork	0.214
R-free	0.26170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2esk 1ubq
RMSD bond length	0.003
RMSD bond angle	0.660
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.710	3.420
High resolution limit [Å]	3.200	3.200
Rmerge	0.089	0.702
Rpim	0.054	0.422
Number of reflections	10704	1931
<I/σ(I)>	8.9	1.5
Completeness [%]	99.7	99.9
Redundancy	3.7	3.7
CC(1/2)	0.996	0.704

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	0.1M MES monohydrate, 14% PEG 4000